đŸ”Ŧ Chimeric Detective Report

Comprehensive Analysis of Chimeric Contigs in Viral Metagenomic Assembly

28
Contigs Analyzed
0
Contigs Split
0
Contigs Preserved
0.0
Mean Confidence

📊 Summary Visualizations

Chimera Type Distribution

Confidence Score Distributions

Plot file not found: confidence_distribution.html

Decision Summary

Evidence Types Overview

🔍 Detailed Analysis Results

Contig ID Chimera Type Confidence Decision Breakpoint Evidence Types Explanation

📈 Individual Contig Details

Click on the links below to view detailed analysis for each chimeric contig:

ℹī¸ Methodology & Interpretation

Detection Methods

Chimeric contigs are detected using multiple complementary approaches:

  • Coverage Discontinuities: Sharp changes in read coverage depth
  • Sequence Composition: Changes in GC content and k-mer frequencies
  • Taxonomic Classification: Transitions between different viral/host lineages
  • Read Pair Orientation: Inconsistent paired-end read orientations

Classification Categories

  • Technical Artifacts: Assembly errors, typically split
  • PCR Chimeras: Amplification artifacts, typically split
  • Biological Recombination: Genuine recombination events, preserved
  • Provirus Integration: Virus-host integration sites, flagged

Confidence Scores

Confidence scores range from 0-1, with higher scores indicating stronger evidence for the classification. Scores above 0.8 are considered high confidence, 0.5-0.8 medium confidence, and below 0.5 low confidence.